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NewEast/Rab3 Activation Assay Kit [82601][20 Assays]/82601

NewEast/Rab3 Activation Assay Kit [82601][20 Assays]/82601
  • NewEast/Rab3 Activation Assay Kit [82601][20 Assays]/82601
商品介绍

ProductHighlight

  • Configuration-specificmonoclonalantibodythatrecognizesRab3-GTP,butnotRab3-GDP
  • TheonlyavailableRab3activationassaykitdirectlymeasuresthelevelofRab3-GTP
  • Providingshorterexperimentaltime
  • CouldbeusedtomonitortheactivationofRab3incellsandintissuesbyimmunohistochemistry

ProductDescription

    Rab3belongstotheRabfamilyofsmallGTPases.Rab3islocatedinthelateendosome,lysosome,cytoplasmicvesicle,phagosomeandmelanosome.Itisinvolvedinthelateendocytictransportandcontributestothematurationofphagosomes.

    CurrentlythereisnodirectassaytomeasuretheactivationofRab3GTPases.

    NewEastBiosciencesRab3ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab3-GTP,butnotRab3-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

    Theseanti-Rab3-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofRab3incellsandintissuesbyimmunohistochemistry.

    NewEastBiosciencesRab3ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab3.Eachkitprovidessufficientquantitiestoperform20assays.

    AssayPrinciple

      NewEastBiosciencesRab3ActivationAssayKitbasesontheconfiguration-specificanti-Rab3-GTPmonoclonalantibodytomeasuretheactiveRab3-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab3activationassays.Briefly,anti-activeRab3mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab3-GTP.TheboundactiveRab3willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab3willbedetectedbyimmunoblotanalysisusinganti-Rab3rabbitpolyclonalantibody.

       ProductManual
     
    RelatedProducts
    Catalog#NameSizePriceDataSheet
    82601Rab3ActivationAssayKit 20assays $637   
    26920ActiveRab3-GTPMonoclonalAntibody 30μL $495   
    品牌介绍

    NewEastBio我们已经开发了一项专有技术(正在申请专利),以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白(至少10倍)。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是,我们保留将抗体作为我们的产品销售的权利。

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    • 收起>>

    Publications:
    1.  VASPpromotesTGF-βactivationofhepaticstellatecellsbyregulatingRab11dependentplasmamembranetargetingofTGF-βreceptors    HepatologyVolume61,Issue1,pages361–374,January2015
    2.  Rab5ActivityRegulatesGLUT4SortingintoInsulin-ResponsiveandNon-Insulin-ResponsiveEndosomalCompartments:APotentialMechanismforDevelopmentofInsulinResistance    Endocrinology.2014Sep;155(9):3315-28