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ProductDescriptionRab3belongstotheRabfamilyofsmallGTPases.Rab3islocatedinthelateendosome,lysosome,cytoplasmicvesicle,phagosomeandmelanosome.Itisinvolvedinthelateendocytictransportandcontributestothematurationofphagosomes. CurrentlythereisnodirectassaytomeasuretheactivationofRab3GTPases. NewEastBiosciencesRab3ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab3-GTP,butnotRab3-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Theseanti-Rab3-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofRab3incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRab3ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab3.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRab3ActivationAssayKitbasesontheconfiguration-specificanti-Rab3-GTPmonoclonalantibodytomeasuretheactiveRab3-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab3activationassays.Briefly,anti-activeRab3mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab3-GTP.TheboundactiveRab3willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab3willbedetectedbyimmunoblotanalysisusinganti-Rab3rabbitpolyclonalantibody. ![]()
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