货号：26309
抗体名：CTNNB1(S45Y)抗体
抗体英文名：CTNNB1(S45Y)antibody
靶点：Beta-catenin,CTNNB,Cateninbeta-1
浓度：1mg/ml
应用范围：ELISA,WB,IHC
宿主：Mouse
供应商：NewEastBio
数量：30000
级别：实验级
目录编号：26309
抗原来源：Mouse
保质期：两年
适应物种：脊椎动物
标记物：FITC
克隆性：是
保存条件：-20℃
形态：液体
亚型：IgG
免疫原：AsyntheticpeptidefromtheinternalregionofCTNNB1whichincludesthemutationofS45Y,humanorigin.
规格：100μl
CTNNB1(S45Y)[26309]
[100ul]		 
CatalogNumber:  26309
GeneSymbol:  Beta-catenin,CTNNB,Cateninbeta-1
Description:  Anti-CTNNB1(S45Y)MouseMonoclonalAntibody
Background:  CTNNB1proteinisadualfunctionprotein.Itisasubunitofacomplexofproteinsthatfromadherensjunctions,whichareimportantfortheestablishmentandmaintenanceofepithelialcelllayersbyregulatingcellgrowthandadhesionbetweenadjacentcells.CTNNB1proteinalsopullsdoubledutyasanintracellularsignaltransducerintheWntsignalingpathway.MutationsofCTNNB1havebeenimplicatedinthepathogenesisofseveralcancers.
Immunogen:  AsyntheticpeptidefromtheinternalregionofCTNNB1whichincludesthemutationofS45Y,humanorigin.
Applications:  ELISA,WB,IHC
RecommendedDilutions:  
ELISA:1:1000-1:2000 
WB:1:500-1:1000 
IHC:1:50-1:100
Concentration:  0.5mg/ml
Host:  Mouse
Format:  Liquid
Clonality:  Monoclonal
Isotype:  
Purity:  Purifiedfromascites
Storagebuffer:
Preservative:  no
Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol
SpeciesReactivity:  RecognizesS45Ymutant,butnotwild-typeCTNNB1ofvertebrates.
StorageConditions:   Storeat-20°C.Avoidfreeze/thawcycles
DownloadDatasheet:  
Westernblot:  
WesternblotanalysisofrecombinantCTNNB1(S45Y)andwildtypeproteins.PurifiedHis-taggedCTNNB1(S45Y)protein(lane2)andcorrespondingwild-typeprotein(lane1)wereblottedwithanti-CTNNB1(S45Y)monoclonalantibody(Cat.#26309).
Immunofluorescence:  
ImmunofluorescenceofcellsexpressingCTNNB1proteinswithanti-CTNNB1(S45Y)antibody.HEK293TcellsweretransfectedwithpCDNA3-GFP-CTNNB1(S45Y)plasmid,pCDNA3-GFP-CTNNB1(WT)plasmidorpCDNA3-GFP-CTNNB1(S45F)plasmid,thenfixedandstainedwithanti-CTNNB1(S45Y)monoclonalantibody(Cat.#26309).
 

 

温馨提示：不可用于临床治疗。