NewEast/Monoclonal Anti-cGMP EIA Kit without Acetylation [80104][5x96 well]/80104,NewEastBio,,NewEastBio,NewEast Biosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：NewEast/Monoclonal Anti-cGMP EIA Kit without Acetylation [80104][5x96 well]/80104

产品编号：80104

市场价：¥31900.00

场地：美国(厂家直采)

产品分类：试剂盒>ELISA试剂盒>特色ELISA试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1595.00

品牌： NewEastBio

公司：NewEast Biosciences

公司分类：

商品介绍

ProductHighlight

BasedontheuniquemousemonoclonalAnti-cGMPantibodyItistheonlyEIAkitonthemarketwithmonoclonalanti-cGMPantibody(allotherkitsarebasedonpolyclonalanti-cGMPantibodies).
Nomorehazardouschemical(aceticanhydride)foryourcGMPassayThemonoclonalAnti-cGMPantibodyhassimilaraffinitiesfornon-acetylatedcGMPandacetylatedcGMP.Thusacetylationwiththeaceticanhydrideispermanentlyeliminatedfromtheassay.
SupersensitivityandselectivityThemonoclonalAnti-cGMPantibodydisplays>10⁸foldofselectivityovercAMP,GTP,andothernucleosideanalogues.TheAnti-cGMPEIAKitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodiesonthemarket.
Shortestassaytimeandhigh-throughputformatTheAnti-cGMPEIAKitisthebestchoicefordrugscreenings.

ProductDescription

Guanosine3",5"-cyclicmonophosphate(cyclicGMP;cGMP)playscriticalregulatoryrolesinmanyphysiologicalprocesses.cGMPisproducedfromGTPbyguanylylcyclasesandisdegradedbyphosphodiesterases.StimulationofguanylylcyclasesorinhibitionofphosphodiesterasescanincreasecellularcGMPconcentrations.InhibitorsofthecGMP-specificphosphodiesterasesareusedfortreatinghumandiseases.Forexample,inhibitorsofcGMPspecificphosphodiesterasetype5(suchasViagraandCialis)areusedfortreatingerectiledysfunction.

Toscreenforinhibitorsofphosphodiesterasesorstimulatorsofguanylylcyclases,itisessentialtohaveafast,sensitive,selectiveandreproducIBLemethodtomeasurethecGMPconcentrations.Thisisespeciallytruefortheinitialscreeningsgiventhepossibleweakereffectsoflargerpoolsofcompounds.

CurrentlyavailableotherELISAkitsmeasuringcGMPlevelsarebasedonthenon-affinity-purifiedpolyclonalanti-cGMPantibody.Despitetheclaimedselectivity,thesepolyclonalanti-cGMPantibodiesdisplaycertaincross-reactivitywithcAMPorGTP.Inmostcelltypes,cGMPispresentatlevels~100foldlowerthancAMP.

NewEastBiosciencescGMPELISAkitisbasedontheuniquemousemonoclonalanti-cGMPantibody.Thismonoclonalanti-cGMPantibodydisplays>108foldofselectivityovercAMP,GTP,andothernucleosideanalogues.NewEastBiosciencescGMPELISAkitprovidessignificantlyimprovedsensitivityandselectivityoverotherkitsbasedonpolyclonalanti-cGMPantibodies.Ourmonoclonalanti-cGMPantibody-basedELISAkitalsoavoidsthebatch-to-batchvariationsassociatedwithpolyclonalantibodyproductionsfromanimals,thusprovidingthereproducibilityinthelongrun.

FurThermore,whilepolyclonalanti-cGMPantibodiesusedinotherELISAkitshavehigheraffinityforacetylatedcGMPthannon-acetylatedcGMP,NewEastBiosciencesmonoclonalanti-cGMPantibodyhassimilaraffinitiestonon-acetylatedandacetylatedcGMPmolecules.Therefore,acetylationtreatmentsofsamplesandstandardsarenotneededinNewEastBiosciencescGMPELISAkit.Thissignificantlyreducesthetimefortheassay.Theavoidanceoforganicreagentsusedintheacetylationprocessprovidesasafeandhealthyworkenvironment.

PrincipleOutline

NewEastBiosciencescGMPELISAKitisacompetitiveimmunoassaytomeasurethecGMPlevels,eitherfromcellextractsorfrominvitroguanylylcyclaseassays.Briefly,multi-wellplatesarecoatedwithgoat-anti-mouseserum.cGMPincellextractsorininvitroguanylylcyclaseassayswillcompetitivelybindtothemonoclonalanti-cGMPantibodyinthepresenceoffixedamountsofcGMP-conjugatedhorse-rADIshperoxidase.KnownamountsofcGMPareusedasstandardstogeneratethecalculationcurve.Afterashortincubation,theexcessreagentsarewashedawayandsubstrateisadded.Themultiwellplatesarethenreadonamicroplatereaderat450nm.TheintensityoftheyellowcolorisinverselyproportionaltotheconcentrationofcGMPinsamples.ThemeasuredopticaldensityisusedtocalculatetheconcentrationofcGMPinsamplesbasedonthecurvefromthecGMPstandards.

ProductManual

AssayLayoutSheet

品牌介绍

NewEastBio我们已经开发了一项专有技术（正在申请专利），以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白（至少10倍）。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是，我们保留将抗体作为我们的产品销售的权利。

公司介绍

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产品分类

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Publications:

1. Sulfhydryl-dependentdimerizationofsolubleguanylylcyclasemodulatestherelaxationofporcinepulmonaryarteriestonitricoxide PflugersArch.2013Feb;465(2):333-41

2. Pressure-overload-inducedsubcellularrelocalization/oxidationofsolubleguanylylcyclaseintheheartmodulatesenzymestimulation CircRes.2012Jan20;110(2):295-303

3. Natriureticpeptidesblocksynaptictransmissionbyactivatingphosphodiesterase2AandreducingpresynapticPKAactivity ProcNatlAcadSciUSA.2012Oct23;109(43):17681-6

4. Roleofsulfhydryl-dependentdimerizationofsolubleguanylylcyclaseinrelaxationofporcinecoronaryarterytonitricoxide CardiovascRes.2011Jun1;90(3):565-72

5. Molt-inhibitinghormonefromChinesemittencrab(Eriocheirsinensis):Cloning,tissueexpressionandeffectsofrecombinantpeptideonecdysteroidsecretionofYOs GenCompEndocrinol.2011Sep15;173(3):467-74

6. InhibitionofStriatalSolubleGuanylylCyclase-cGMPSignalingReversesBasalGangliaDysfunctionandAkinesiainExperimentalParkinsonism PLoSOne.2011;6(11):e27187

7. Molecularanalysis,developmentalfunctionandheavymetal-inducedexpressionofABCC5inzebrafish CompBiochemPhysiolBBiochemMolBiol.2011Jan;158(1):46-55

8. FeedbackcontrolthroughcGMP-dependentproteinkinasecontributestodifferentialregulationandcompartmentationofcGMPinratcardiacmyocytes CircRes.2010Nov12;107(10):1232-40

9. TheSolubleGuanylateCyclaseActivatorBAY58-2667ProtectsagainstMorbidityandMortalityinEndotoxicShockbyRecouplingOrganSystems PLoSOne.2013Aug28;8(8):e72155

10. Stimulationofguanylylcyclase-Dbybicarbonate Biochemistry.2009May26;48(20):4417-22

11. Volumeoverloadinducesdifferentialspatiotemporalregulationofmyocardialsolubleguanylylcyclaseineccentrichypertrophyandheartfailure JournalofMolecularandCellularCardiologyVolume60,July2013,Pages72–83

12. DefiningSpecificityDeterminantsofCyclicGMP-MediatedGustatorySensoryTransductioninCaenorhaBDitiselegans genetics.113.152660v1194/4/885

13. Theroleofnitricoxidesignalinginfoodintake;insightsfromtheinnermitochondrialmembranepeptidase2mutantmice RedoxBIOLOGyVolume1,Issue1,2013,Pages498–507

14. CrossregulationbetweencGMP-dependentproteinkinaseandAktinvasodilatationofporcinepulmonaryartery JournalofCardiovascularPharmacology:November2014-Volume64-Issue5-p452-459

15. Geneticablationofsolutecarrierfamily7a3aleadstohepaticsteatosisinzebrafishduringfasting HepatologyVolume60,Issue6,pages1929–1941,December2014