NewEast/Rap1B Protein [10120][100 µg]/10120,NewEastBio,,NewEastBio,NewEast Biosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：NewEast/Rap1B Protein [10120][100 µg]/10120

产品编号：10120

市场价：¥4780.00

场地：美国(厂家直采)

产品分类：蛋白类>蛋白纯化>蛋白纯化试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$239.00

品牌： NewEastBio

公司：NewEast Biosciences

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商品介绍

Catalog Number: 10120

Product Name: Rap1B Protein

Synonyms: Member of RAS oncogene family, K-REV, RAL1B

Source: Human, recombinant full length, His6-tag

Expression Host: E. coli

Molecular Weight: 21 kDa

Purity: >95% by SDS-PAGE

Introduction: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway.

Amino Acid Sequence (1-184)

MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDAQQCMLEILDTAGTEQFTAM RDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTDDVPMILVGNKCDLEDERVVGKEQGQNL ARQWNNCAFLESSAKSKINVNEIFYDLVRQINRKTPVPGKARKKSSCQLL

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Rap1B was determined by SDS- PAGE and Coomassie Brilliant Blue Staining

References: hrzanowska-Wodnicka, M. et al., J. Clin. Invest. 115: 680-687, 2005. 2. Hoshijima, M. et al., Biochem. Biophys. Res. Commun. 157: 851-860, 1988. 3. Kawata, M. et al., Proc. Nat. Acad. Sci. 87: 8960-8964, 1990. 4. Kawata, M. et al., J. Biol. Chem. 264: 15688-15695, 1989. 5. Lova, P. et al., J. Biol. Chem. 278: 131-138, 2003. 6. Matsui, Y. et al., Biochem. Biophys. Res. Commun. 166: 1010-1016, 1990. 7. Pizon, V. et al., Nucleic Acids Res. 16: 7719-only, 1988. 8. Rehmann, H. et al., Nature 455: 124-127, 2008. 9. Rousseau-Merck, M. F. et al., Cytogenet. Cell Genet. 53: 2-4, 1990. 10. Schwamborn, J. C. et al., Nature Neurosci. 7: 923-929, 2004.

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品牌介绍

NewEastBio我们已经开发了一项专有技术（正在申请专利），以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白（至少10倍）。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是，我们保留将抗体作为我们的产品销售的权利。

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