CatalogNumber: 21272 |
GeneSymbol: EGFR |
Description: Anti-EGFRRabbitPolyclonalAntibody |
Background: EGFRisareceptortyrosinekinaseforligandsoftheEGFfamily.EGFRactivatesseveralsignalingcascadestoconvertextracellularcuesintoappropriatecellularresponses.DefectsinEGFRareassociatedwithcancerssuchaslungcancer. |
Immunogen: PurifiedEGFRprotein,humanorigin. |
Applications: ELISA,WB,IHC |
RecommendedDilutions: ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200 |
Concentration: 0.2mg/ml |
Host: Rabbit |
Format: Liquid |
Clonality: Polyclonal |
Isotype: |
Purity: Purifiedfromserum |
Storagebuffer: |
Preservative: no |
Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol |
SpeciesReactivity: RecognizesEGFRofvertebrates. |
StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles |
DownloadDatasheet: |
Westernblot: |
WesternblotanalysisofrecombinantEGFRprotein.PurifiedHis-taggedEGFRproteinwasblottedwithanti-EGFRrabbitpolyclonalantibody(Cat.#21272). |
Immunohistochemistry: |
Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancer(upper)andlungcancer(lower)tissuewithanti-EGFRpolyclonalantibody(Cat.#21272).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-mouseIgG(1:50dilution)wasusedassecondaryantibody. |
Publications:1. Immunohistochemistrywithanovelmutation-specificmonoclonalantibodyasascreeningtoolfortheEGFRL858Rmutationalstatusinprimarylungadenocarcinoma TumorBIOLOGy.October2014 | 品牌介绍
NewEastBio我们已经开发了一项专有技术(正在申请专利),以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白(至少10倍)。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是,我们保留将抗体作为我们的产品销售的权利。
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