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℡ 4000-520-616
℡ 4000-520-616
NewEast/EGFR [21272][100 µL]/21272
产品编号:21272
市  场 价:¥4380.00
场      地:美国(厂家直采)
产品分类: 抗体类>一抗>多克隆抗体>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$219.00
品      牌: NewEastBio
公      司:NewEast Biosciences
公司分类:
NewEast/EGFR [21272][100 µL]/21272
商品介绍

CatalogNumber:  21272
GeneSymbol:  EGFR
Description:  Anti-EGFRRabbitPolyclonalAntibody
Background:  EGFRisareceptortyrosinekinaseforligandsoftheEGFfamily.EGFRactivatesseveralsignalingcascadestoconvertextracellularcuesintoappropriatecellularresponses.DefectsinEGFRareassociatedwithcancerssuchaslungcancer.
Immunogen:  PurifiedEGFRprotein,humanorigin.
Applications:  ELISA,WB,IHC
RecommendedDilutions:  ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200
Concentration:  0.2mg/ml
Host:  Rabbit
Format:  Liquid
Clonality:  Polyclonal
Isotype:  
Purity:  Purifiedfromserum
Storagebuffer:
Preservative:  no
Constituents:  PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol
SpeciesReactivity:  RecognizesEGFRofvertebrates.
StorageConditions:  Storeat-20°C.Avoidfreeze/thawcycles
DownloadDatasheet:  
Westernblot:  
WesternblotanalysisofrecombinantEGFRprotein.PurifiedHis-taggedEGFRproteinwasblottedwithanti-EGFRrabbitpolyclonalantibody(Cat.#21272).
Immunohistochemistry:  
Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancer(upper)andlungcancer(lower)tissuewithanti-EGFRpolyclonalantibody(Cat.#21272).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-mouseIgG(1:50dilution)wasusedassecondaryantibody.
品牌介绍

NewEastBio我们已经开发了一项专有技术(正在申请专利),以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白(至少10倍)。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是,我们保留将抗体作为我们的产品销售的权利。

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Publications:
1.  Immunohistochemistrywithanovelmutation-specificmonoclonalantibodyasascreeningtoolfortheEGFRL858Rmutationalstatusinprimarylungadenocarcinoma    TumorBIOLOGy.October2014