| CatalogNumber: 21272 |
| GeneSymbol: EGFR |
| Description: Anti-EGFRRabbitPolyclonalAntibody |
| Background: EGFRisareceptortyrosinekinaseforligandsoftheEGFfamily.EGFRactivatesseveralsignalingcascadestoconvertextracellularcuesintoappropriatecellularresponses.DefectsinEGFRareassociatedwithcancerssuchaslungcancer. |
| Immunogen: PurifiedEGFRprotein,humanorigin. |
| Applications: ELISA,WB,IHC |
| RecommendedDilutions: ELISA:1:1000-1:5000WB:1:100-1:1000IHC:1:50-1:200 |
| Concentration: 0.2mg/ml |
| Host: Rabbit |
| Format: Liquid |
| Clonality: Polyclonal |
| Isotype: |
| Purity: Purifiedfromserum |
| Storagebuffer: |
| Preservative: no |
| Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol |
| SpeciesReactivity: RecognizesEGFRofvertebrates. |
| StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles |
DownloadDatasheet: ![]() |
| Westernblot: |
![]() | WesternblotanalysisofrecombinantEGFRprotein.PurifiedHis-taggedEGFRproteinwasblottedwithanti-EGFRrabbitpolyclonalantibody(Cat.#21272). |
| Immunohistochemistry: |
![]() |
![]() |
| Immunohistochemicalanalysisofparaffin-embeddedhumanthyroidcancer(upper)andlungcancer(lower)tissuewithanti-EGFRpolyclonalantibody(Cat.#21272).Tissuesampleswerefixedwithformaldehydeandblockedwith1%serumfor15minat37°C.Antigenretrievalwasbyheatmediationincitratebuffer(pH6).Sampleswerethenincubatedwithprimaryantibody(1:50dilution)overnightat4°C.AHRP-conjugatedGoatanti-mouseIgG(1:50dilution)wasusedassecondaryantibody. |
Publications:| 1. Immunohistochemistrywithanovelmutation-specificmonoclonalantibodyasascreeningtoolfortheEGFRL858Rmutationalstatusinprimarylungadenocarcinoma TumorBIOLOGy.October2014 | 品牌介绍
NewEastBio我们已经开发了一项专有技术(正在申请专利),以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白(至少10倍)。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是,我们保留将抗体作为我们的产品销售的权利。 ![]()
厂家直采
全球直采 正品优价
![]()
正品保障
厂家直发 有线跟踪
![]()
正规清关
CIF100%正规报关,提供发票
![]()
及时交付
限时必达 不达必赔
|