货号: | 28001 |
抗体名: | c-Myc抗体 |
抗体英文名: | c-Mycantibody |
靶点: | c-Myc |
浓度: | 1mg/ml |
宿主: | Mouse |
供应商: | NewEastBio |
数量: | 30000 |
级别: | 生化级 |
目录编号: | 28001 |
抗原来源: | Mouse |
保质期: | 两年 |
适应物种: | 脊椎动物 |
标记物: | FITC |
克隆性: | 是 |
保存条件: | -20℃ |
形态: | 液体 |
亚型: | IgG |
规格: | 100μl |
cMyc Catalog Number: 28001 Gene Symbol: CMYC Description: c-Myc mouse Monoclonal Antibody
Background: c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence specific binding function. Immunogen: c-Myc (9E10) is a mouse monoclonal antibody epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human origin. Tested applications: IP, IHC c-Myc (9E10) is recommended for detection of c-Myc p67 of mouse, rat and human origin by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), immunoprecipitation [1–2 mg per 100–500 mg of total protein (1 ml of cell lysate)], immunofluorescence and immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 mg per 1 x 106 cells); non cross-reactive with N-Myc or L-Myc proteins Concentration: 1 mg/ml Host: mouse Clonality: Monoclonal Isotype: IgG Purity: Purified from ascites Format: Liquid Storage buffer: Preservative: no Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |