Catalog Number: 26213 | Gene Symbol: PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha | Description: Anti-PIK3CA (Q546P) Mouse Monoclonal Antibody | Background: Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer. | Immunogen: A synthetic peptide from the internal region of PIK3CA which includes the mutation of Q546P, human origin. | Applications: ELISA, WB | Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:2000 | Concentration: 1 mg/ml | Host: Mouse | Format: Liquid | Clonality: Monoclonal | Isotype: | Purity: Purified from ascites | Storage buffer: | Preservative: no | Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol | Species Reactivity: Recognizes Q546P mutant, but not wild-type PIK3CA of vertebrates. | Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles | Download Datasheet: | Western blot: |
Western blot analysis of recombinant PIK3CA (Q546P) and wildtype proteins. Purified His-tagged PIK3CA (Q546P) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-PIK3CA (Q546P) monoclonal antibody (Cat. #26213). |
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