cMyc
Catalog Number: 28001
Gene Symbol: CMYC
Description: c-Myc mouse Monoclonal Antibody
Background: c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc
proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and
colon carcinomas while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small
cell lung carcinomas.
The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence specific binding function.
Immunogen: c-Myc (9E10) is a mouse monoclonal antibody epitope corresponding to amino acids 408-439 within the C-terminal domain of c-Myc of human origin.
Tested applications: IP, IHC
c-Myc (9E10) is recommended for detection of c-Myc p67 of mouse, rat and human origin by Western Blotting (starting dilution 1:200,
dilution range 1:100-1:1000), immunoprecipitation [1–2 mg per 100–500 mg of total protein (1 ml of cell lysate)], immunofluorescence and
immunohistochemistry (including paraffin-embedded sections) (starting dilution 1:50, dilution range 1:50-1:500) and flow cytometry (1 mg per 1 x 106 cells); non cross-reactive with N-Myc or L-Myc proteins
Concentration: 1 mg/ml
Host: mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
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