CatalogNumber: 26214 | GeneSymbol: PIK3CA,CLOVE,CWS5,MCAP,MCM,MCMTC,PI3K,p110-alpha | Description: Anti-PIK3CA(Q546R)MouseMonoclonalAntibody | Background: Phosphatidyl3-kinases(PI3K)areafamilyoflipidkinasesinvolvedinmanycellularprocesses,includingcellgrowth,proliferation,differentiation,motility,andsurvival.Itiscomposedofan85kDaregulatorysubunitanda110kDacatalyticsubunit.MutantsofPIK3CAhasbeenfoundinthepathogenesisofseveralcancers,includingcoloncancer,gliomas,gastriccancer,breastcancer,endometrialcancer,andlungcancer. | Immunogen: AsyntheticpeptidefromtheinternalregionofPIK3CAwhichincludesthemutationofQ546R,humanorigin. | Applications: WB | RecommendedDilutions: ELISA:1:1000-1:5000 WB:1:500-1:2000 | Concentration: 1mg/ml | Host: Mouse | Format: Liquid | Clonality: Monoclonal | Isotype: | Purity: Purifiedfromascites | Storagebuffer: | Preservative: no | Constituents: PBS(withoutMg2+andCa2+),pH7.4,150mMNaCl,50%glycerol | SpeciesReactivity: RecognizesQ546Rmutant,butnotwild-typePIK3CAofvertebrates. | StorageConditions: Storeat-20°C.Avoidfreeze/thawcycles | DownloadDatasheet: | Westernblot: |
 | WesternblotanalysisofrecombinantPIK3CA(Q546R)andwildtypeproteins.PurifiedHis-taggedPIK3CA(Q546R)protein(lane2)andcorrespondingwildtypeprotein(lane1)wereblottedwithanti-PIK3CA(Q546R)monoclonalantibody(Cat.#26214). |
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