NewEast/Ras Protein [10110][100 µg]/10110,NewEastBio,,NewEastBio,NewEast Biosciences,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：NewEast/Ras Protein [10110][100 µg]/10110

产品编号：10110

市场价：¥4780.00

场地：美国(厂家直采)

产品分类：蛋白类>蛋白纯化>蛋白纯化试剂盒>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$239.00

品牌： NewEastBio

公司：NewEast Biosciences

公司分类：

商品介绍

Catalog Number: 10110

Product Name: Ras Protein

Synonyms: GTPase Ras

Source: Human, recombinant full length, His6-tag

Expression Host: E. coli

Molecular Weight: 21 kDa

Purity: >95% by SDS-PAGE

Introduction: Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

Amino Acid Sequence (1-189)

MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAM RDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLA RSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS

Properties

Physical Appearance (form): Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.

Physical Appearance (form): White or clear

Concentration: 1 mg/mL

Storage: -80°C

Preparation Instructions: Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside (DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His-tagged Ras was determined by SDS- PAGE and Coomassie Brilliant Blue Staining.

References: 1. Chen, H.-J. et al., Neuron 20: 895-904, 1998. 2. Hamdan, F. F. et al., New Eng. J. Med. 360: 599-605, 2009. 3. Oh, J. S. et al., Neuron 33: 151 only, 2002. 4. Rumbaugh, G. et al., Proc. Nat. Acad. Sci. 103: 4344-4351, 2006. 5. Tomoda, T. et al., Genes Dev. 18: 541-558, 2004.

Datasheet:

Publications:

1. DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model Gene Therapy 21, 888-896 (October 2014)

品牌介绍

NewEastBio我们已经开发了一项专有技术（正在申请专利），以生产与GTP结合的G蛋白的单克隆抗体。抗体应比结合GDP的蛋白更好地识别纯化的结合GTP的G蛋白（至少10倍）。我们可以利用该技术为您的特殊G蛋白生成抗体。活性G蛋白抗体的成本为10,000美元。但是，我们保留将抗体作为我们的产品销售的权利。

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